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Friday, December 26, 2008

Classification of Viruses

Hierarchy of Classification

All living things are grouped into 3 domains which are Archea, Eukaryotes and Prokaryotes which are further divided into Kingdom, Phylum, Class, Order, Family, Genus and Species based on similarities which they have with each other. The diagram below shows the hierarchy of classification of living things using Humans (Homo sapiens) as an example.

The name given to each organism is given using its genus name followed by its species name. The first letter of the name is usually capitalized and the whole name is either italicized or underlined, for instance humans are named: Homo sapiens or Homo sapiens.


Virus Classification

Viruses only display characteristics of living entities only when they are within a host cell and possess unique structural and genetic features. As a result it is not classified in the same way that typical living organisms are.

There are two schemes of classification of viruses; they are Lwoff’s Scheme and Baltimore’s Scheme. These two schemes are discussed briefly below.


Lwoff’s Scheme for Classification

This scheme developed by Lwoff, RW Horne and P Tournier in 1962 make use of shared properties of viruses rather than the host cell or organism they infect. The classification for viruses under this scheme consist of phylum, class, order, family, sub family, genus, species and strain/type.

Four main characteristics used to group the viruses are:

1) Nature of the nucleic acid (DNA or RNA)
2) Capsid symmetry
3) Presence or absence of an envelope
4) Viron and Capsid Dimensons

The international committee on Taxonomy of Viruses (ICTV) has accepted and is using certain principles in this scheme in addition to that of Baltimore’s scheme.


Baltimore’s Scheme for Classification

This scheme developed by David Baltimore uses mechanisms of viral genome replication and the principle that all virus genomes need to construct positive strand RNA from their genomes in to produce virus proteins for replication to categorize viruses.

Various types of virus genomes have different mechanisms for replication and these genomes are grouped into 7 groups which are shown in the diagram below:

(click image to enlarge)
Baltimore’s classification uses the way the different groups of genetic material are processed into RNA. The diagram below shows how the virus’s genetic materials are converted to mRNA and the intermediate processes and products involved.

(click image to enlarge)
Fig 1: Flowchart showing the conversion of different groups of genetic materials to mRNA and then to proteins


ICTV Taxonomy

The ICTV uses both Baltimore’s and Lwoff’s schemes of classifications for viruses and classifies viruses based on the following:

1) virus genome and sequence relatedness,
2) structure of the virus,
3) natural host range,
4) cell and tissue tropism,
5) pathogenicity and cytopathology,
6) mode of transmission,
7) physiochemical properties
8) and antigenic properties of viral proteins

Presently virus classification is still under development as a result some viruses may not have been assigned to an order, family or genus yet. As a result, informal grouping based on ICTV taxonomic ranking, genome structure and composition is used to classify them.

Type Species

Under the International Code of Virus Taxonomy and Nomenclature classification, each genus is typified using a species, in other words, each genus name will be linked to one species name. This species name linked to the genus is known as the type species.

In this system, each genus will contain a type species assigned to it. The link between the type species and the genus will not always be based on characteristics, some maybe be historical, defining the origins of the genus name. As a result, type species may not be a typical member of the genus and may not characterize the genus.

The table below gives examples of the type species of certain genus.



(click image to enlarge)
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Tuesday, December 23, 2008

Introduction to Virology
Virology and Its Importance

Virology is a branch of microbiology that studies viruses and diseases caused by them.

The study of viruses is vital as many fatal diseases are caused by viruses such as AIDs, Hepatitis and Dengue Haemorrhagic Fever and are the cause of several epidemics and pandemics that killed large numbers of people such as the recent SARS outbreak. In addition viruses also play an important role in research by being used as vectors of genetic material in the study of genetics. Since viruses posses such important biological roles and can be used as a tool for further technological advancement, hence it is viable and essential that it is studied and researched upon.


Fig 1: Hepatitis B Viron

Fig 2: 1918 Influenza Pandemic



Brief History of Biology

Viruses were not closely studied till 1898 when a Russian Scientist, Martinus Beijernick discovered that the tobacco mosaic disease was caused by a filterable agent smaller than that of viruses. Hence the Tobacco Mosaic Virus became the first known virus to be discovered.

Before the discovery of viruses, methods to combat it were already developed. For instance, in 1885 in Europe Edward Jenner developed the first vaccine which was used to combat the small pox virus. He realized that milkmaids who had cowpox did not contract smallpox at all and realized that cow pox could be used as a vaccine.

The table below shows a rough timeline of the important events of Virology till 1898.

(Click Image to Magnify)

Definition of Viruses

Viruses are described as infectious obligate intracellular parasites possessing the following structural features:

(Click Image to Magnify)

Viruses have different shapes and sizes and can be classified into different categories based on that. The categories are Helical, Icosahedral, Enveloped, and Complex. The picture below shows how a typical virus from the respective categories above looks like.

Fig 3: Helical Virus

Fig 5: Enveloped Virus

Fig 4: Icosahedral Virus
Fig 6: Complex Virus


Videos

These video clips shows how viruses infect cells and how they make use of the host cell’s machinery to replicate.










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References:

Pictures:
·Fig1:http://sg.wrs.yahoo.com/_ylt=A0S0zu9OXk9JJC4AOIIu4gt./SIG=12q3mcovb/EXP=1230024654/**http%3A/www.vfa.de/img/db/hepatitis_b_virus_16857295.jpg_175_177_60.jpg
· Fig2: http://pd.ilt.columbia.edu/banneker/fgproj/fgimages/fnsthsp1_s.jpg
· Fig3:http://media-2.web.britannica.com/eb-media/44/4944-004-332E2643.gif
· Fig4: http://www.public-health.uiowa.edu/adv/pics/Adeno1.gif
· Fig5: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/viruses/images/u2fig2d.jpg
· Fig6: http://www.nsf.gov/od/lpa/news/02/images/bacteriophage.jpg

Videos:
· http://www.youtube.com/watch?v=gU8XeqI7yts&feature=related
· http://www.youtube.com/watch?v=A409yO-G1Mk&feature=related

Information:
· http://www.kcom.edu/faculty/chamberlain/website/lects/PROPERT.HTM#gen
· http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20102/bio%20102%20lectures/viruses/viruses.htm




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